rebase

Documentation

Overview

Package rebase for parsing the rebase restriction enzyme database

Index

• func Parse(rebaseRh io.Reader) []wtype.RestrictionEnzyme

Functions

func Parse

func Parse(rebaseRh io.Reader) []wtype.RestrictionEnzyme

Parse a database of restriction enzyme data in the structure of a rebase database into a set of RestrictionEnzymes. Data must be structured in the following format:

<1><name> <2><prototype> <3><recognition sequence> <4><methylation site> <5><commercial source> <6><reference>

REBASE codes for commercial sources of enzymes

B        Life Technologies (5/16)
C        Minotech Biotechnology (5/16)
E        Agilent Technologies (3/16)
I        SibEnzyme Ltd. (5/16)
J        Nippon Gene Co., Ltd. (5/16)
K        Takara Bio Inc. (5/16)
M        Roche Applied Science (5/16)
N        New England Biolabs (5/16)
O        Toyobo Biochemicals (8/14)
Q        Molecular Biology Resources - CHIMERx (5/16)
R        Promega Corporation (3/16)
S        Sigma Chemical Corporation (5/16)
V        Vivantis Technologies (8/14)
X        EURx Ltd. (3/16)
Y        SinaClon BioScience Co. (5/16)

e.g.

<1>AaaI <2>XmaIII <3>C^GGCCG <4> <5> <6>1680